Biochemical Test of Escherichia coli (E. coli)
| Basic Characteristics | Properties (E. coli) |
| Capsule | Capsulated |
| Catalase | Positive (+ve) |
| Citrate | Negative (-ve) |
| Coagulase | Negative (-ve) |
| Flagella | Flagellated |
| Gas | Positive (+ve) |
| Gelatin Hydrolysis | Negative (-ve) |
| Gram Staining | Negative (-ve) |
| Growth in KCN | Negative (-ve) |
| H2S | Negative (-ve) |
| Hemolysis | Negative (-ve) |
| Indole | Positive (+ve) |
| Motility | Motile |
| MR (Methyl Red) | Positive (+ve) |
| Nitrate Reduction | Positive (+ve) |
| OF (Oxidative-Fermentative) | Fermentative |
| Oxidase | Negative (-ve) |
| Pigment | Negative (-ve) |
| PYR | Negative (-ve) |
| Shape | Rod |
| Spore | Non-Sporing |
| TSIA (Triple Sugar Iron Agar) | Acid/Acid, Gas +ve |
| Urease | Negative (-ve) |
| VP (Voges Proskauer) | Negative (-ve) |
Fermentation
| Adonitol | Negative (-ve) |
| Arabinose | Positive (+ve) |
| Arabitol | Negative (-ve) |
| Cellobiose | Negative (-ve) |
| DNase | Negative (-ve) |
| Dulcitol | Variable |
| Erythritol | Negative (-ve) |
| Glucose | Positive (+ve) |
| Glycerol | Variable |
| Lactose | Positive (+ve) |
| Malonate | Negative (-ve) |
| Maltose | Positive (+ve) |
| Mannitol | Positive (+ve) |
| Mannose | Positive (+ve) |
| Melibiose | Positive (+ve) |
| Mucate | Positive (+ve) |
| Myo-Inositol | Negative (-ve) |
| Raffinose | Variable |
| Rhamnose | Positive (+ve) |
| Salicin | Variable |
| Sorbitol | Positive (+ve) |
| Sucrose | Variable |
| Tartrate | Positive (+ve) |
| Trehalose | Positive (+ve) |
| Xylose | Positive (+ve) |
Enzymatic Reactions
| Acetate Utilization | Positive (+ve) |
| Aesculine Hydrolysis | Variable |
| Arginine Dihydrolase | Negative (-ve) |
| Beta Lactamase | Positive (+ve) |
| Esculin Hydrolysis | Variable |
| Lipase | Negative (-ve) |
| Lysine Decarboxylase | Positive (+ve) |
| ONPG (galactosidase) | Positive (+ve) |
| Ornithine Decarboxylase | Variable |
| Phenylalanine Deaminase | Negative (-ve) |
